Impact of an online course on enhancing the diagnosis of Chagas disease in Latin America

ABSTRACT Objective. Chagas disease poses a public health problem in Latin America, and the electrocardiogram is a crucial tool in the diagnosis and monitoring of this pathology. In this context, the aim of this study was to quantify the change in the ability to detect electrocardiographic patterns among healthcare professionals after completing a virtual course. Materials and Methods. An asynchronous virtual course with seven pre-recorded classes was conducted. Participants answered the same questionnaire at the beginning and end of the training. Based on these responses, pre and post-test results for each participant were compared. Results. The study included 1656 participants from 21 countries; 87.9% were physicians, 5.2% nurses, 4.1% technicians, and 2.8% medical students. Initially, 3.1% answered at least 50% of the pre-test questions correctly, a proportion that increased to 50.4% after the course (p=0.001). Regardless of their baseline characteristics, 82.1% of course attendees improved their answers after completing the course. Conclusions. The implementation of an asynchronous online course on electrocardiography in Chagas disease enhanced the skills of both medical and non-medical personnel to recognize this condition.


Introduction
Chagas disease is highly prevalent in Latin America, mainly associated with poverty and adverse socioeconomic conditions (1) .However, recent migratory trends have led to the global dissemination of this disease, generating increasing interest in high-income regions and countries (1)(2)(3)(4) .t is estimated that approximately 1 out of every 3 individuals infected with Trypanosoma cruzi will develop cardiac involvement over their lifetime, with a latency period of several decades from the initial infection to the disease (2,5,6) .
The electrocardiogram (ECG) is a valuable tool in the evaluation of individuals at risk of Chagas disease due to its low cost and wide availability.Therefore, it is recommended to perform an annual ECG in individuals with positive serology and without heart disease (3,7,8) , since electrocardiographic abnormalities are related to complications and worse prognosis in this condition (6,(9)(10)(11) .
Despite being a public health issue in Latin America, the care for Chagas disease has been neglected for decades (1) .To address this gap, the Inter-American Society of Cardiology (SIAC) implemented a virtual course in Spanish in 2023 with the aim of raising awareness about the disease, encouraging early suspicion and diagnosis, and improving skills in electrocardiographic evaluation among different healthcare team.In this context, the aim of the present study was to quantify the change in the ability to detect electrocardiographic patterns by healthcare professionals after completing a virtual course.

Statistical analysis
An exploratory descriptive analysis was conducted where the variables were analyzed as mean and standard deviation, assessing normality with graphical tools (histograms and normal probability plots) and the Shapiro-Wilk test.Categorical variables were expressed in absolute values and percentages.
For the comparison of pre-and post-test responses, McNemar's test was used for paired samples.The internal consistency of the test questions was analysed with Cronbach's alpha coefficient.
Finally, bivariate regression analysis explored associations between baseline characteristics and knowledge acquisition through the course.For this purpose, logistic regression analysis was carried out between the dependent and independent variables.

Ethical aspects
The study complied with the Declaration of Helsinki and international ethical guidelines for biomedical research.
Confidentiality and data protection were guaranteed, and only the principal investigator had access to personal information.
In contrast, all data processing and analysis was conducted anonymously, ensuring confidentiality and protecting the identity of each participant.The project was approved by the SIAC Executive Committee.

Results
The study included 1,656 participants from 21 countries (Table 1), with an average age of 36.2 ± 10.9 years; 60.3% were men.Of the participants, 87.9% were physicians; among these, 37.1% were cardiologists, 17.4% were internal medicine specialists, 25.0% were family doctors, 6.4% reported having no specialty, 1.7% were pediatricians (including pediatric cardiologists), and the remaining 12.4% had other specialties.Among the 12.1% of nonphysician participants, 5.2% were nurses, 4.1% were cardiology practice technicians, and 2.8% were medical students.
The average rate of correct answers among participants was 23.0% ± 11.7% in the pre-test, with no differences between medical and non-medical personnel (22.9% vs. 23.0%,respectively, p=0.910).The percentage of correct answers on the post-test was 55.2% ± 23.4%, with a similar response rate between medical and non-medical participants (p=0.988).No significant differences were observed between medical and non-medical personnel when analyzing each individual response of the pre-test (Table 2).

Study design and population
To assess the impact of the course on participants' knowledge, a non-controlled before-and-after study was conducted using two multiple-choice exams.Both medical and non-medical personnel from Latin America were invited to participate in the course, with no age or profession restrictions.Upon registration for the course, participants were asked to declare their area of work or training.
Additionally, medical personnel were asked to specify their specialty, if applicable.Due to the nature of the program, nonprobabilistic convenience sampling was used.

Intervention
A virtual and asynchronous course was developed, with free and open access, on an educational platform dedicated specifically for this purpose (https://www.siacardio.com/academia/ecgchagas/).
The main objective of the course was to increase knowledge about ECG for the early detection of Chagas cardiomyopathy among various healthcare team members.To achieve this, seven theoretical classes were created that covered general aspects of the disease, its incidence, prevalence, the importance of early diagnosis, as well as the treatment and care of people living with this disease.Five of the seven modules specifically focused on the different manifestations and alterations of the ECG in this condition.In addition, a virtual library with supplementary materials was made available to the students, expanding on all aspects covered in the course.
The course began in May 2023 and continued until December of the same year.All participants had unrestricted access to the complete course material throughout the period, without any limit on class viewing.
Each enrolled participant answered a ten-question questionnaire, with four possible options and only one correct answer.At the end of the seven modules of the course, the students had to answer the same exam as the beginning, but with the questions and options presented randomly to prevent mechanical answering.

Study variables
The dependent variable was whether or not the participant correctly answered 50% or more of the post-test exam questions, and the independent variables were gender, age, the role of the participants in the healthcare system, and, among the doctors, their specialties.The p-values correspond to the result of the McNemar test for comparing each pair of questions.
3.1% of the course participants correctly answered at least 50% of the pre-test exam questions, a proportion that increased to 50.4% after the course (p=0.001).The increase in the proportion of correct answers occurred across all questions of the questionnaire (p<0.001; Figure 1).82.1% of the participants answered one or more questions correctly in the post-test evaluation compared to their performance before the course.On the other hand, 9.9% of the participants had the same number of correct answers after the course; finally, 8.0% of the course attendees performed worse in the post-test exam compared to the pre-test evaluation.
No differences were found in the baseline characteristics of the participants and the improvement in answers in the post-test exam (Table 3).Because of this, the creation of a multivariable model was not performed.
Finally, the internal consistency of the questionnaire items was assessed using Cronbach's alpha test, yielding a Cronbach's alpha value of 0.60, suggestive of good consistency among the questionnaire items.

Discussion
The main findings of the present study are: i) an asynchronous online e-learning course enhancing the abilities to detect various characteristics and electrocardiographic patterns in Chagas disease; and ii) the positive impact of the course was similar among medical and non-medical personnel, as well as among professionals from different specialties.
More than a decade ago, it was pointed out that the rapid advancement of scientific knowledge in Medicine poses significant challenges for professionals seeking to keep up-to-date (12) .
Although precise data do not exist, it is estimated that, in the 1950s, medical knowledge would double in 50 years, whereas by 2010, this duration had been reduced to 3.5 years (12) .Currently, it is believed that the timeframe for the duplication of available biomedical knowledge has been shortened to less than a year.This presents a substantial challenge for healthcare professionals across all fields.
Technological advances have enabled the implementation of new educational tools, such as augmented or virtual reality, distance learning via interactive platforms, the use of social networks, and, more recently, the integration of artificial intelligence into the teaching-learning process (13)(14)(15) .
Furthermore, its simple and rapid acquisition allows tracings to be obtained by non-medical personnel trained in this task, making it the ideal method for screening a large number of individuals , especially those with difficulties accessing complex healthcare systems, even in suburban and rural areas (1)(2)(3) .For this reason, it was decided to include non-medical personnel in the program.
Similarly to the present study, other surveys conducted in the region have shown that there are topics inadequately addressed in the training of physicians across different specialties (16)(17)(18) .However, this work goes further by not just identifying a problem but by presenting an innovative alternative to cope with it.In our study, a brief self-administered educational program for participants had the capacity to Statistical significance was established as a value of p<0.05 in two-tailed tests for all tests performed.Analyses were performed using Stata version 18.0 (Stata Corp., College Station, TX, USA).

Figure 1 .
Figure 1.Percentage of correct answers in each question of the exam before (pretest) and after the course (posttest).Los valores de p corresponden al resultado de la prueba de McNemar para la comparación de cada par de preguntas.

Table 1 .
Country of residence of study participants

Table 2 .
Proportion of correct answers of medical and non-medical participants in the pre-test examThe p-values correspond to the McNemar's test result.

Table 3 .
Baseline characteristics of participants, and improvement in post-test responses 95% CI: 95% confidence interval.